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KMID : 0613820120220050697
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Journal of Life Science
2012 Volume.22 No. 5 p.697 ~ p.701
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Effect of Activated Protein C (APC) on Apoptosis of Cancer Cells
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Min Kyoung-Jin
Bae Jong-Sup
Kwon Taeg-Kyu
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Abstract
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Activated protein C (APC) has an anticoagulant effect and a non-hemostatic effect such as regulation of cell metastasis and modulation of inflammation. In this study, we investigated whether APC could modulate apoptosis in cancer cells. Tumor necrosis factor (TNF)-α, cyclohexamide, and FAS markedly induced apoptosis in human renal carcinoma Caki cells. When Caki cells were pretreated with APC, the percentage of death receptor-induced apoptosis did not change. Furthermore, we checked the effect of APC on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human glioma T98G and human breast carcinoma MDA231 cells. APC also had no effect on TRAIL-induced apoptosis in both cell lines. However, pretreatment with APC inhibited combination treatment (kahweol plus TRAIL and kahweol plus melatonin)-induced apoptosis and PARP cleavage in Caki cells. Taken together, our results suggest that APC can modulate anti-cancer therapeutic efficiency.
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KEYWORD
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Activated protein C (APC), apoptosis, TRAIL, Caki cells, kahweol
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